In order to investigate the prevalence of swine influenza virus (SIV) in Shandong, China, a pig farm in Binzhou, Shandong, was investigated. Nasal swab samples suspected of containing SIV were collected and inoculated into Specific Pathogen Free (SPF) chicken embryos for virus isolation.  Subsequently, the isolated virus underwent whole-genome sequencing, genetic evolution analysis, protein key amino acid mutations analysis, and evaluation of pathogenicity to piglets. The results showed that a strain of SIV H1N1 subtype was successfully isolated and named as A/swine/Shandong/BZ03/2022 (H1N1), known as SIV H1N1 SDBZ strain. Sequence alignment analysis indicated that the HA and NA gene of the isolate exhibited the highest homology with A/swine/Liaoning/DL1007/2020 (H1N1) in GenBank, with similarities of 99.41 and 99.57%, respectively. The amino acid sequence of the HA protein cleavage site was identified as PSIQSR$GLF, displaying low pathogenic molecular characteristics. The pathogenicity experiment demonstrated that infected piglets exhibited influenza symptoms such as fever, runny nose, and sneezing. SIV was consistently detected in nasal swabs of piglets, and led to meat-like consolidation in the lungs. Pathological examinations revealed parenchymal lungs with disappeared alveoli. Therefore, this study successfully isolated a H1N1 subtype SIV strain in Shandong Province, elucidating its genetic evolution, molecular characteristics, and pathogenicity. This work provides some important references for the monitoring and prevention and surveillance of SIV in China.