The emergence of antibiotic resistance owing to the imprudent use of antibiotics in food-producing animals has been associated with huge costs and threats, particularly in countries with comparable farming practices like Pakistan. The present study evaluated the efficacy of 10 antibiotics using their standard concentrations against Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum and Pullorum from three districts of Punjab province in Pakistan. Surprisingly, 66.7% of the isolates were resistant to the β-lactam potentiator, amoxicillin-clavulanate. On the contrary, 23.3% and 33% of the isolates were resistant to the two III-generation aminobenzyl penicillins, amoxicillin and ampicillin, respectively. 56.6% of isolates were found to be resistant to the II-generation aminoglycoside Gentamicin, and 100% resistance was observed against Nalidixic Acid, Ciprofloxacin, or Levofloxacin. A high degree of susceptibility to Sulfamethoxazole-Trimethoprim and Doxycycline was observed. The antibiotics exhibited statistical significance, via correlation and cluster analysis. Conclusively, the study reports the multitude of multidrug resistance in host-adapted Salmonella, emphasizing the importance of continued research and monitoring into the use of antibiotics in agro-food animals on a national level.