PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2024, 44(3): xxx

First Report of Zoonotic Cryptosporidium parvum Subtype IIaA15G2R1 in Dogs in Türkiye

Adnan Ayan¹*, Burcak Aslan Celik², Ozgur Yasar Celik³, Gurkan Akyildiz⁴, Ozlem Orunc Kilinc⁵, Ozge Oktay Ayan⁶, Fatma Ertas Oguz⁷, Yasar Goz⁸, Nazmi Yuksek⁹, Ali Bilgin Yilmaz¹⁰, Assem Myrzhiyeva¹¹, Aizhan Akhmetzhanova¹² and Ugur Uslu¹³

¹Department of Genetics, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye.²Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye.³Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye.⁴Department of Basic Health Sciences, Health Sciences Faculty, Marmara University, Istanbul, Türkiye.⁵Ozalp Vocational School, Van Yuzuncu Yil University, Van, Türkiye.⁶Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye.⁷Tuzluca Vocational School, Igdir University, Igdir, Türkiye.⁸Department of Nutrition and Dietetics, Faculty of Health Sciences, Van Yuzuncu Yil University, Van, Türkiye.⁹Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye.¹⁰Faculty of Health, Van Yuzuncu Yil University, Van, Türkiye.¹¹Veterinary Sciences, Kazakh National Agrarian Research University, Kazakhstan¹²Department of Veterinary, Faculty of Agrarian, Shakarim State University of Semey, Kazakhstan.¹³Department of Parasitology, Faculty of Medicine, University of Istanbul Medeniyet, Istanbul, Türkiye
*Corresponding author: dradnanayan@gmail.com

Abstract

Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, Türkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyoun's acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in Türkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces.

To Cite This Article: AyanA, CelikBA, CelikOY, AkyildizG, KilincOO, AyanOO, OguzFE, GozY, YuksekN, YilmazAB, MyrzhiyevaA, AkhmetzhanovaA, and UsluU, 2024. First report of zoonotic Cryptosporidium parvum subtype IIaA15G2R1 in dogs in Türkiye. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2024.274

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)