In the current study, we explored the ability of Cordyceps militaris (CM) mushroom powder to prevent hyperlipidemia in albino rats fed a high-cholesterol diet (HCD). Twenty-five male Albino Wister rats were divided into 5 groups, each consisting of five rats. PC (positive control group) was kept on a high-cholesterol diet, while rats in the negative control group (NC) were fed a standard basal diet and distilled water. The standard group (STD) (treatment group) received a dose of 10 mg/kg of body weight of Simvastatin. The low dose (LD) and high dose (HD) groups were administered CM powder, at 12g/kg b.wt. and 24 g/kg b.wt. /day, respectively, along with the basal diet, given orally daily for eight weeks.  Our results clearly showed that CM significantly reduced the levels of TC, TG, and LDL levels by 55.65 %, 56.32 %, and 38.49 %, in LD respectively, and by 63.40 %, 64.08%, and 66.39 % respectively in HD. CM consumption significantly increased the value of HDL by 52.81 % and 57.45 % in LD and HD respectively. There was a significant decrease in serum MDA (Malondialdehyde) and serum TOS (Total Oxidative Stress) levels in the treatment groups of LD and HD_, however, the level of serum catalase (CAT) and serum TAC (Total Antioxidant Capacity) followed an increasing trend in these groups. Moreover, CM at high doses triggered a significant drop in the amounts of aspartate transaminase (AST), and alanine transaminase (ALT) enzymes as compared to the NC by 27.2 %, and 34.82 % respectively. Furthermore, the normal histological structure was restored in the HD as compared to the PC group. Our results revealed that a high dose of CM could prevent HCD-induced hyperlipidemia and have the potential to be used as a functional food ingredient.