Cryptosporidium (C.) parvum is the most prevalent species in the middle-east countries especially in Iraq and its zoonotic importance is not well documented. Hence, the current work included the study of the genetic profile of Cryptosporidium species from the fecal samples of infected cattle owners and cattle. Two hundred stool specimens were collected from cattle owners complaining of watery diarrhea and 100 fecal samples from diarrheic cattle in several rural areas of Zakho district. All stool specimens were analyzed microscopically using modified Ziehl Neelsen stain technique and genetically using traditional PCR approach targeting 18S rRNA, HSP70 and gp60 genes. The prevalence of Cryptosporidiosis in humans was 70% (140/200) and among infected cattle was 62% (62/100) by using modified Ziehl-Neelsen stain. On other hand, the molecular analysis (PCR) identified Cryptosporidiosis in 74.5% (149/200) among human samples and 65% (65/100) among cattle samples. The similarity in the analysis of Targeting genes presented that all isolates were identified as C. parvum which have 82–99.8 % similarity to other isolates from Iraq, China and USA. The phylogenetic analysis based on gp60 sequences of Cryptosporidium parvum revealed two subtypes belonging to family subtypes IIa (IIdA21G1 and IIdA19G1). This study confirms the prevalence of C. parvum among humans and cattle in this area. Furthermore, the C. parvum isolated from cattle are genetically identical to those found in humans.