Salmonellosis is a significant public health concern globally, primarily caused by the consumption of environmentally driven contaminated food and water. Salmonella enterica can infect human and animals via associated environment, that makes this bacterium one of the potential one health pathogens. Regrettably, irrational antimicrobial use (AMU) in food producing animals (FPAs) like poultry creates selection pressure that leads to the emergence of resistance against critically important antibiotics for human medicine such as colistin.  In this study, Salmonella enterica serovar Typhimurium isolates were recovered from poultry and their associated environments. Confirmation and antibiotic resistance profiling of the isolates was done through standard microbiological procedures and clinical and laboratory standards institute (CLSI) guidelines. Whole-genome sequencing (WGS) was employed to assess the genomic features of the S. enterica isolates, with a focus on identifying antimicrobial resistance and virulence genes. Phylogenetic relationships were analyzed through comparative genomics and whole-genome multi-locus sequence typing (wgMLST). The results revealed a significant distribution (23%) of S. Typhimurium, whereas 8% among them were colistin resistant. The isolated strain exhibited unique genetic outlook compared to previously reported genomic characteristics. Notably, unlike other S. enterica genomes from poultry-related environments, this isolate contained a complete phage (Sal3) integrated into its genome. Several virulence genes and antibiotic resistance genes (ARGs) were identified, responsible for the distinct pathogenic ability of S. enterica. The present study displayed a detailed genomic vista of S. enterica, that may be useful for the future interventions to control the infections caused by this one health pathogen.

To Cite This Article: Mahmood S, Rasool MH, Khurshid M, Aslam B, 2025. Genetic outlook of Colistin resistant Salmonella enterica Serovar Typhimurium recovered from Poultry-Environment Interface: A One Health Standpoint. Pak Vet J, 45(1): 246-256. http://dx.doi.org/10.29261/pakvetj/2025.118