Genetic outlook of Colistin resistant
Salmonella enterica
Serovar Typhimurium recovered from Poultry-Environment Interface: A
One Health Standpoint
Sara Mahmood, Muhammad Hidayat Rasool, Mohsin Khurshid and Bilal
Aslam*
Institute of Microbiology, Government College University Faisalabad,
Faisalabad 38000, Pakistan
*Corresponding author:
drbilalaslam@gcuf.edu.pk
Abstract
Salmonellosis is a significant public health concern globally, primarily caused
by the consumption of environmentally driven contaminated food and water.
Salmonella enterica can infect human and animals via associated environment,
that makes this bacterium one of the potential one health pathogens.
Regrettably, irrational antimicrobial use (AMU) in food producing animals (FPAs)
like poultry creates selection pressure that leads to the emergence of
resistance against critically important antibiotics for human medicine such as
colistin.In this study, Salmonella
enterica serovar Typhimurium isolates were recovered from poultry and their
associated environments. Confirmation and antibiotic resistance profiling of the
isolates was done through standard microbiological procedures and clinical and
laboratory standards institute (CLSI) guidelines. Whole-genome sequencing (WGS)
was employed to assess the genomic features of the S. enterica isolates,
with a focus on identifying antimicrobial resistance and virulence genes.
Phylogenetic relationships were analyzed through comparative genomics and
whole-genome multi-locus sequence typing (wgMLST). The results revealed a
significant distribution (23%) of S. Typhimurium, whereas 8% among them
were colistin resistant. The isolated strain exhibited unique genetic outlook
compared to previously reported genomic characteristics. Notably, unlike other
S. enterica genomes from poultry-related environments, this isolate
contained a complete phage (Sal3) integrated into its genome. Several virulence
genes and antibiotic resistance genes (ARGs) were identified, responsible for
the distinct pathogenic ability of S. enterica. The present study
displayed a detailed genomic vista of S. enterica, that may be useful for
the future interventions to control the infections caused by this one health
pathogen.
To Cite This Article:
MahmoodS, RasoolMH, KhurshidM, Aslam
B, 2025. Genetic outlook of Colistin
resistant Salmonella enterica Serovar
Typhimurium recovered from Poultry-Environment Interface: A One Health
Standpoint. Pak Vet J.
http://dx.doi.org/10.29261/pakvetj/2025.118