PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2025, 45(1) 440-444

Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on ITS1-5.8S rRNA from Van Province, Turkey

Ali Bilgin Yilmaz^1*, Erkan Azizoglu², Özdemir Adizel³, Yaşar Göz¹, Burcak Aslan celik⁴, Ozgur Yasar CELİK⁵, Ozge Oktay Ayan⁶, Aizhan Akhmetzhanova⁷, Adnan Ayan⁸, Muhammed Yasul⁹, Assem Myrzhiyeva¹⁰ and Ugur Uslu¹¹

¹Faculty of Health, Van Yuzuncu Yil University, Van, Türkiye.²Department of Plant and Animal Production, Colemerik Vocational School, Hakkari University, 30100, Hakkari, Türkiye.³Department of Biology, Faculty of Science, Van Yuzuncu Yıl University, 65080, Van, Türkiye⁴Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye⁵Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye⁶Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye⁷Department of Veterinary, Faculty of Agrarian, Shakarim State University of Semey, Kazakhstan.⁸Department of Parasitology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Türkiye⁹Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye.¹⁰Veterinary Sciences, Kazakh National Agrarian Research University, Kazakhstan¹¹Department of Parasitology, Faculty of Medicine, University of Istanbul Medeniyet, Istanbul, Türkiye

*Corresponding author: alibilginyilmaz@yyu.edu.tr

Abstract

This study aimed to calculate the occurence of Moniezia species in cattle, sheep, and goats in Van province and to identify these species using morphological and molecular methods (ITS1-5.8S rRNA gene region analysis). Additionally, the study aimed to identify the genetic differences between Moniezia expansa and Moniezia benedeni. During the summer of 2022, intestinal contents were collected from 150 ruminants (50 cattle, 50 sheep, and 50 goats) slaughtered in slaughterhouses in Van province. The parasites were examined using Aceto-Carmine staining, and species identification were based on interproglottidal glands. Examination of the intestinal contents revealed that 2 out of 50 cattle (4%), 14 out of 50 sheep (28%), and 9 out of 50 goats (18%) were infected with Moniezia. Morphological and molecular analyses showed that the cattle samples were identified as Moniezia benedeni, goats samples as Moniezia expansa, and those from sheep as 11 Moniezia expansa and 3 Moniezia benedeni. Following DNA extraction, the ITS1-5.8S rRNA gene region was amplified using PCR and subjected to sequence analysis. The relationship between species was examined by phylogenetic tree. This study confirms the prevalence of Moniezia spp. in Van/Türkiye by using the ITS1-5.8S rRNA gene.

To Cite This Article: YILMAZ AB, Azizoglu E, Adizel O, Göz Y, Celik BA, Celik OY, Ayan OO, Akhmetzhanova A, Ayan A, Yasul M, Myrzhiyeva A and Uslu U, 2025. Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on ITS1-5.8S rRNA from Van Province, Turkey. Pak Vet J, 45(1): 440-444. http://dx.doi.org/10.29261/pakvetj/2025.131

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)