Nanoparticles and nanocomposites having potential applications in different areas are widely used in industrial and biomedical fields. Cellular inflammatory response due to exposure to nanoparticles can be monitored by measuring of inflammatory biomarkers/oxidative stress in different tissues of exposed animals. Oxidative stress parameters, contents of antioxidant enzymes and histopathological changes in erythrocytes and testicular tissues were assessed to determine the cell membrane integrity in male albino rats following 5, 10 and 15mg/kg body weight intraperitoneal administration of nanocomposites (vanadium, magnesium and nickel) daily for a period of 15 days. A dose and time dependent increase in contents of lipid peroxidation and reactive oxygen species (ROS) as well as a significant decrease in reduced glutathione (GSH) and the contents of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were measured using biochemical analysis. Statistical analysis showed significant (P<0.05) variation in contents of different biomarkers of erythrocytes and testes in treated and untreated rats. Histopathological observations showed prominent testicular damage in term of degeneration of seminiferous tubules, detachment of germinal epithelium, edema and Leydig cell degeneration in rats treated with high dose depicting impaired process of spermatogenesis and reproductive toxicity. These results indicate that prolonged exposure to vanadium, magnesium and nickel nanocomposites cause significant oxidative stress and histopathological ailments. It is suggested that measuring of oxidative stress and status of antioxidant enzymes are reliable tools to determine the induction of inflammatory response in cells.

To Cite This Article: Abduallah AM, Aslam Z, Aldawood N, Sindi RA, Mahmood Y, Mustafa G and Ahmed AE, 2025. Investigation of Toxic Effects of Vanadium, Magnesium and Nickel Nanocomposite on Erythrocytes and Reproductive Organ of Male Albino Rats. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.125