PAKISTAN VETERINARY JOURNAL

Pak Vet J, xxxx, xx(x) xxx-xxx

Investigating the effectiveness of doum and marjoram powders as dietary supplements in mitigating the negative effects of ochratoxin on broiler chickens health

Esraa M. Fahmy¹, Emad Adeeb Ibrahem¹, Gamal Eldin Amin Shams¹, Eslam Hamed¹, Tarek khamis¹, Layla A. Almutairi², Kamlah Ali Majrashi³, Diana A.H. Al-Quwaie³, Suad Hamdan Almasoudi⁴, Aiman A. Alsaegh⁵, Saleh Altuwaijri^6*, Ahmed Ezzat Ahmed^7,8, Heba M. Salem^9,10 and Hala M.N. Tolba¹¹

¹Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt; ²Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia; ³Biological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia; ⁴Department of Biology, College of Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia; ⁵Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Saudi Arabia; ⁶Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, P.O. Box 6622, Buraydah, 51452, Saudi Arabia; ⁷Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia; ⁸Prince Sultan Bin Abdelaziz for Environmental Research and Natural Resources Sustainability Center, King Khalid University, Abha 61421, Saudi Arabia; ⁹Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University; Giza, 12211, Egypt; ¹⁰Department of Diseases of Birds, Rabbits, Fish & their Care & Wildlife, School of Veterinary Medicine, Badr University in Cairo (BUC), Badr City, 11829, Cairo, Egypt; ¹¹Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

*Corresponding author: stoiejry@qu.edu.sa

Abstract

Mycotoxins pose a significant risk to poultry production as they have detrimental effects on chicken health, immunity, and productivity. Additionally, they present a hazard to human health due to their teratogenic and carcinogenic properties. This work investigated the influence of using doum palm fruit and marjoram leaves' powders separately or in combination against experimentally induced ochratoxicosis in broiler chicks. A total of 100 day-old Cobb chicks were divided into 5 equal groups (20 chick/group with duplicate) as follows: G1: control negative birds, kept on feeding with starter broiler ration free from ochratoxin; G2: control positive birds, fed starter broiler ration with ochratoxin @ 5.4ppb; G3: fed starter broiler ration with ochratoxin @ 5.4ppb and treated with doum fruit powder @ 100g/kg ration; G4: fed starter broiler ration with ochratoxin @ 5.4ppb and treated with marjoram powder @ 15g/kg ration, and G5: fed starter broiler ration with ochratoxin @ 5.4ppb and treated with doum fruit powder @ 100g/kg ration and marjoram powder @ 15g/kg. The dual use of doum and marjoram powder at 15g/kg reduced the adverse effects by improving the renal and hepatic functions and reducing the histopathological as well as immunohistochemical alterations within liver, kidney, spleen, and heart tissues. Also, a significant improvement in the humoral immune response had been detected as elucidated by expression of different vital genes such as TBP, OCLN, MUC-1, JAM-2, CD4, CD8, IL-1b, IL6, TLR-4, BAX, Casp-3, and Gapdh; along with improvement in the antioxidant status. In conclusion, doum fruit powder (100g/kg) and marjoram powder (15 g/kg) can be used as feed additives to reduce the adverse effects induced by ochratoxicosis in broiler chickens.

To Cite This Article: Fahmy EM, Ibrahem EA, Shams GEA, Hamed E, Khamis T, Almutairi LA, Majrashi KA, Al-Quwaie DAH, Almasoudi SH, Alsaegh AA, Altuwaijri S, Ahmed EA, Salem HM, Tolba HMN, 2025. Investigating the effectiveness of doum and marjoram powders as dietary supplements in mitigating the negative effects of ochratoxin on broiler chickens health. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.145

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)