Morin Inhibits Inflammation, Oxidative Stress and Ferroptosis in
Lipopolysaccharide-Induced Endometritis
Aftab Shaukat1,2, Shahid Ali Rajput3, Sana
Hanif4, Rizwan Shukat5, Mehboob Ali2, Irfan
Shaukat6, Mubashar Hassan2, Muhammad Tahir
Aleem7, Muhammad Mohsin8, Shu-cheng Huang9
and Renwei Su1*
1College
of Veterinary Medicine, South China Agricultural University,
Guangzhou, China; 2College of Veterinary Medicine,
Huazhong Agricultural University, Wuhan, China; 3Faculty
of Veterinary & Animal Sciences, MNS-University of Agriculture,
Multan, Pakistan; 4Hubei Key Laboratory of Theory and
Application of Advanced Materials Mechanics, Wuhan University of
Technology, Wuhan, China; 5Faculty of Food, Nutrition &
Home Sciences, University of Agriculture, Faisalabad, Pakistan;
6Department of Biochemistry, University of Narowal, Narowal,
Pakistan; 7Department of Pharmacology, Shantou University
Medical College, Shantou, China; 8Fujian University of
Traditional Chinese Medicine, Fujian, Fuzhou, China; 9College
of Veterinary Medicine, Henan Agricultural University, Zhengzhou,
China.
*Corresponding author:
renweisu@scau.edu.cn
Abstract
Endometritis is a uterine condition that results in significant detriment to the
advancement of the global dairy industry.
Morin is a phyto-flavonoid compound known for its diverse biological functions.
The effect of morin on LPS-triggered endometritis remained undetermined. In the
current study, sixty adult mice were allocated
to
four (n = 15) treatment groups, i.e., Control, LPS, LPS + Morin, and
Morin groups.
Intrauterine
infusion of LPS was used to establish an endometritis model in mice. An
intraperitoneal injection of morin was administered to the mice 1 hour before an
intrauterine
infusion of LPS. After 24h of
intrauterine injection of
LPS, all the mice were euthanized. Our results revealed that morin rescued
uterine injury by relieving
the
histopathological alterations caused by LPS.
Morin
significantly (P<0.05) reduced
myeloperoxidase and pro-inflammatory cytokines
following
LPS exposure. It significantly (P<0.05) attenuated malondialdehyde production
and iron accumulation, while markedly enhanced glutathione and adenosine
triphosphate levels in response to LPS exposure. Furthermore, morin treatment
significantly increased mRNA and protein expression of TLR4. Moreover, it
significantly (P<0.05) repressed TLR4 and down streamed NF-κB signalling.
Notably, morin (P<0.05)
suppressed LPS-induced oxidative damage by scavenging ROS and enhancing
antioxidant enzymes activity.
Additionally, morin activated the Nrf2 pathway, that was downregulated by LPS,
which enhanced the antioxidant defence system. Our findings demonstrated that
morin confers protection against endometritis in mice by inhibiting
inflammation, oxidative stress and ferroptosis.
To Cite This Article:
Shaukat A, Rajput SA, Hanif S, Shukat R, Ali M, Shaukat I, Hassan M, Aleem MT,
Mohsin M, Huang SC and Su R. 2025.
Morin inhibits Inflammation, Oxidative stress and Ferroptosis in
Lipopolysaccharide-induced Endometritis.
Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.171