Probiotics have emerged as a natural and effective alternative to antibiotics due to their relevancy to gut health and immune booster functions both in animals and humans. In this study, five indigenous Streptomyces strains—Streptomyces rochie, Streptomyces fimbriatus, Streptomyces WSN2, Streptomyces globiosporus, and Streptomyces toxytricin—were selected for molecular characterization and in vitro evaluation of their probiotic potential. These strains were isolated from native soil samples and identified using 16S rRNA gene sequencing. Each strain was screened for key probiotic traits, including resistance to pH, antimicrobial activity against common pathogens, and compatibility with standard antibiotics. The results indicated that all five strains exhibited notable tolerance to gastrointestinal-like conditions, with S. WSN2 and S. fimbriatus showing the most robust antimicrobial effects.. In conclusion, the selected indigenous Streptomyces species possess promising traits to be considered as probiotic candidates, with possible applications for the improvement of human and animal health.

To Cite This Article: Sun T, Khaliq S, Liang X, Ashraf M, Ashraf N, Rahman S, Khan I, Abdelraheem MZ and Maqbool B 2025. Molecular characterization and probiotic potential of indigenous streptomyces isolates. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.241