PAKISTAN VETERINARY JOURNAL

Pak Vet J, xxxx, xx(x) xxxx-xxxx

Antimicrobial Susceptibility and Resistance Mechanism of Campylobacter jejuni and Campylobacter coli isolated from Broiler, Turkey and Cattle in Türkiye

Şahin Çakır^1*, Kadir Serdar Diker², Mehmet Akan³, Mustafa Bebek⁴, Muammer Göncüoğlu⁵, Şükrü Kırkan², Gonca Öztap Özen⁴, Güzin Şahin⁴, Erkan Taçbaş⁶, Hafize Tuğba Yüksel Dolgun², Hamit Kaan Müştak³, İnci Başak Müştak³, Özlem Kardoğan⁷, Fethiye Çöven⁸, İrem Gülaçtı⁹, Kamile Kesler¹⁰, Nevin Turut¹¹, Orbay Sayı¹², Şenay Seyitoğlu¹², Özge Yılmaz Çağırgan⁸, Veli Gülyaz¹³, Naim Deniz Ayaz¹⁴, Serap Savaşan², Güzin İplikçioğlu Aral⁵, Muharrem Satılmış¹⁵, Seza Eskiizmirliler⁸, Ayhan Akman¹⁶, Erdem Saka¹⁶, Arzu Ürşen Aşyemez¹⁷, Mehmet Hakan Tabak⁹, Durmuş Kahveci⁴, Halim Kavak⁴, Mustafa Ulucan¹⁸, Seden Arzu Birinci¹⁹, Hülya Sökmen²⁰, Mehmet Levent Kaynar¹⁰, Asuman Soysal Sarışahin¹⁹ and Harun Hasdoğan⁹

¹Department of Microbiology, Faculty of Veterinary Medicine, Necmettin Erbakan University, Konya, 42310, Türkiye; ²Department of Microbiology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Aydın, 09020, Türkiye; ³Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06070, Türkiye; ⁴General Directorate of Food and Control, Ministry of Agriculture and Forestry, Ankara, 06800, Türkiye; ⁵Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06070, Türkiye; ⁶General Directorate of Agricultural Research and Policies, Ministry of Agriculture and Forestry, Ankara, 06800, Türkiye; ⁷Veterinary Control Central Research Institute, Ministry of Agriculture and Forestry, Ankara, 06220, Türkiye; ⁸Bornova Veterinary Control Institute, Ministry of Agriculture and Forestry, İzmir, 35040, Türkiye; ⁹Pendik Veterinary Control Institute, Ministry of Agriculture and Forestry, İstanbul, 34890, Türkiye; ¹⁰Konya Veterinary Control Institute, Ministry of Agriculture and Forestry, Konya, 42090, Türkiye; ¹¹Adana Veterinary Control Institute, Ministry of Agriculture and Forestry, Adana, 01330, Türkiye; ¹²Erzurum Veterinary Control Institute, Ministry of Agriculture and Forestry, Erzurum, 25070, Türkiye; ¹³Department of Virology, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, 63200, Türkiye; ¹⁴Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, 71450, Türkiye; ¹⁵Menemen Vocational School, Bakırçay University, İzmir, 35665, Türkiye; ¹⁶Samsun Veterinary Control Institute, Ministry of Agriculture and Forestry, Samsun, 55200, Türkiye; ¹⁷Food and Feed Control Central Research Institute, Ministry of Agriculture and Forestry, Bursa, 16160, Türkiye; ¹⁸İskilip District Directorate of Agriculture and Forestry, Ministry of Agriculture and Forestry, Çorum, 19400, Türkiye; ¹⁹Ankara Food Control Laboratory Directorate, Ministry of Agriculture and Forestry, Ankara, 06170, Türkiye; ²⁰İzmir Food Control Laboratory Directorate, Ministry of Agriculture and Forestry, İzmir, 35200, Türkiye

*Corresponding author: sahin.cakir@erbakan.edu.tr

Abstract

Campylobacteriosis is a leading zoonotic disease of global public health significance. Antibiotics are still the scientific and realistic way in the treatment of infectious bacterial diseases. However, AMR has been rapidly increasing worldwide due to overuse and misuse of antibiotics. In this study, C. jejuni and C. coli strains isolated from cecal samples of broiler, turkey, and cattle at the EPU level were used. The isolates were tested for susceptibility to critical and indicator antimicrobials based on ECOFF, and resistance mechanisms were analysed. Resistance rates according to species for nalidixic acid, ciprofloxacin, and tetracycline among the analysed Campylobacter isolates were identified as 75.9, 77.9, and 76.8% in broiler isolates; 80.7, 82.2, and 82.2% in turkey isolates; and 62.9, 63.8, and 75.2% in cattle isolates, respectively. In most strains, FQ resistance was primarily associated with a Thr-86-Ala mutation in the gyrA gene, while tetracycline resistance was linked to the tetO gene. Single point mutation in 23S rRNA gene (usually A2075G) or ermB gene was detected in a few strains with erythromycin resistance. In MDR strains, the cmeB membrane efflux gene was identified. In this study, the level of AMR and resistance mechanisms in indicator C. jejuni and C. coli strains isolated from food-producing animals demonstrated the need for urgent and sustainable collaborative action to monitor and control AMR. This study aimed to characterize the national antimicrobial resistance profiles of Campylobacter isolates from broilers, turkeys, and cattle, and to elucidate the underlying resistance mechanisms within a One Health framework.

To Cite This Article: Çakır Ş, Diker KS, Akan M, Bebek M, Göncüoğlu M, Kırkan Ş, Öztap Özen G, Şahin G, Taçbaş E, Yüksel Dolgun HT, Müştak HK, Müştak IB, Kardoğan Ö, Çöven F, Gülaçtı İ, Kesler K, Turut N, Sayı O, Seyitoğlu Ş, Yılmaz Çağırgan Ö, Gülyaz V, Ayaz ND, Savaşan S, İplikçioğlu Aral G, Satılmış M, Eskiizmirliler S, Akman A, Saka E, Ürşen Aşyemez A, Tabak MH, Kahveci D, Kavak H, Ulucan M, Birinci SA, Sökmen H, Kaynar ML, Soysal Sarışahin A and Hasdoğan H, 2026. Antimicrobial susceptibility and resistance mechanism of campylobacter jejuni and campylobacter coli isolated from broiler, turkey and cattle in Türkiye. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2026.014

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)