Bovine papillomaviruses cause a contagious viral disease in cattle known as papillomatosis. This study was conducted to determine the molecular presence of papillomavirus in cattle raised in Konya Province. For this purpose, a total of 25 papilloma lesion samples developing in the head, neck, and teat regions of 25 cattle raised for milk and meat production in the Ahirli, Bozkir and Yalihuyuk districts of Konya Province were collected. These lesions were analyzed at the molecular level using the conventional PCR method with the FAP59/64 and MY11/09 consensus primers. While no positivity was detected in any of the samples analyzed using the FAP59/64 primer pair, positive results were obtained in 2 of the 25 samples (8%) examined with the MY11/09 primer pair. In the second-stage analysis performed using type-specific primers in all samples, Bovine Papillomavirus (BPV) types were detected in 24 of the 25 samples (96%). BPV-3, BPV-5 and BPV-11 types were not detected in any of the samples. The BPV types identified using type-specific primers were determined to be BPV-1, BPV2, BPV-4, BPV-6, BPV-8, BPV-9, BPV-10 and BPV-12. In the overall distribution, BPV-12 (95.83%) was identified as the most prevalent type for the first time. Macroscopic examination revealed that 52% of the lesions were classified as having a typical morphological form. No statistically significant association was found between the macroscopic appearance, anatomical localization of the lesions, and the detected BPV types (P>0.05). In conclusion, various BPV types responsible for papillomatosis were determined to be present in cattle raised in Konya Province.

To Cite This Article: Kaya Y, Bulut O and Kale M, 2026. Molecular detection of bovine papillomaviruses in cattle in konya province: the emergence of BPV-12. Pak Vet J, 46(8): 2025-2031. http://dx.doi.org/10.29261/pakvetj/2026.192