Chicken is a popular and important food poultry; however, little is known about the intestinal microbiota and metabolite differences between Xizang chicken (LH) and flat area chicken (ZJ). Hence, we performed this study to compare the microbiota structure and metabolites of chicken from different altitudes via multi-omics sequencing. Results showed that there were 567 614 filtered sequences in bacterial microbiota and 610 966 filtered sequences in fungal microbiota. The indexes of Pieloue (P<0.05) and Simpson (P<0.05) in bacterial microbiota of the LH group were significantly higher than those in ZJ group. Bacteria biomarkers of Ezakiella, Peptococcus, Faecalicoccus, Limosilactobacillus, Ligilactobacillus, Clostridium T, Akkermansia and fungi biomarker of Pichia were found between the two chicken groups. There were 260 differential metabolites discovered between higher and lower altitude chickens. Our results may give novel insights into future Xizang chicken breeding and understanding their plateau adaptability.

To Cite This Article: Wang H, Xian L, Da P, Wu Y, Su Z, Zang L, Wang H, Zhu Y and Feng J, 2026. Multi-omics comparing analysis of chicken from different altitudes. Pak Vet J, 46(9): 2293-2302. http://dx.doi.org/10.29261/pakvetj/2026.220