Abstract   

Seroprevalence and virus isolation studies were carried out to determine the role of Bovine herpesvirus-l (BHV-l) in reproductive ailments of cattle and huft Serum samples collected from slaughter house Rawalpindi and Livestock Research Station (LRS) were subjected to the indirect immunofluorescence to detect the antibodies against BHV-l. Out of 268 serum samples from slaughter house, 58(21 .64%) were seropositive for BHV-l antibodies. Out of 185 samples from LRS, 53(28.65%) were positive for BHV-l antibodies. The sample from aborted animals & their fetuses were also tested for the presence of IBRV. No virus could be detected from field cases of aborted fetuses both by cell culture moculation and by direct fluorescent antibody test on the tissue samples. The study revealed the seroprevalence of Infectious bovine rhinotracheitis (IBR) in cattle & buffaloes in Pakistan particularly in animals with reproductive disorders.