FFECTS OF DIFFERENT MATURATION AND CULTURE MEDIA
ON IVF OF SHEEP OOCYTES
S. Birler, S. Pabuccuoglu, S. Alkan, K. Ak, M.
Evecen and
I. K. Ileri
Department of Reproduction and Artificial
Insemination, Faculty
of Veterinary Medicine,
University
of
Istanbul, Avcilar-34850,
Turkey
Abstract
This study was performed 1) to compare different
ratios of foetal calf serum (FCS) for in vitro maturation (IVM) of sheep oocytes
and 2) to investigate different culture media and supplements for ovine embryo
production in vitro. Primary oocytes
collected from ovaries of slaughtered Kivircik ewes were divided into 2 groups
randomly and incubated in vitro for 26 hours at 39°C in a humidified atmosphere
of 5% CO2 in air, TCM 199 medium supplemented with 10mg/ml follicle stimulating hormone, 10mg/ml luteinizing hormone, 1 mg/ml estradiol 17β and 10% FCS (low) or 20% FCS (high)
was used as maturation medium. After in vitro maturation and fertilization,
presumptive zygotes were again divided into 2 groups randomly, and co-cultured
for 6 days with sheep oviductal epithelial cells. In the first culture group
(TCM), TCM 199 medium supplemented with 55 sheep estrous serum (SES), and in the
second group synthetic oviduct fluid (SOF) medium supplemented with 20% SES were
used. The cleavage and morula rates among groups (low +TCM, high+TCM, low+SOF,
High+SOF) were 38.8ab, (38/98), 33.3b, (28/84), 52.0a
(53/102) and 53.0 a (44/83); and 22.4ab (22/98), 16.7b
(14/84), 31.4a (32/102) and 34.9%a (29/83),
respectively. Differences among groups with different letters (a, b) were
important statistically (p<0.05). The results of this study showed that in vitro
culture (IVC) of sheep embryos derived in vitro could be better in SOF medium
than TCM 199.