Livestock Production Research Institute,
Bahadurnagar,
Okara,
Pakistan
Abstract
A total of 2340 mammary glands of 585 primiparous
and pluriparous lactating buffaloes in different stages of lactation were
examined with California Mastitis Test ( CMT) and laboratory examination was
carried out to identify the most prevalent micro-organisms in clinical and
sub-clinical mastitis.The physical
examination revealed 2.61 per cent blind teats and CMT revealed 6.71 per cent
positive quarters for mastitis. Microbiological examination of 157 sub-clinical
mastitis milk samples and 46 clinical mastitis milk samples was carried out.There was high occurrence of streptococci (35.46%) followed by
staphylococci (33.99%), E. coli (27.09%), pseudomonas spp. ( 1.97%) and
Corynaebacterium pyogenes (1.48%).The in
vitro sensitivity revealed kanamyciin (82.6%), highly effective against various
isolates followed by gentamycin (53.0%).
Key words: Mastitis,
California
Mastitis Test, Staphylococci, Streptococci.