FECT OF
EXPERIMENT AL
YOLK SAC INFECTION WITH STAPHYLOCOCCUS
AUREUS ON
IMMUNE STATUS
OF BROILERS
M. F. Rai,
S. A. Khan, A. Aslam. K. Saeed, 1K. A. Khan and M. S. Shah
Department of Pathology, 1Department of Parasitology,
University
of
Veterinary
and
Animal, Sciences,
Lahore,
Pakistan.
Abstract
The
present project was carried out to study the effect of experimental yolk sac
infection with Staphylocvccus aureus on immune status of broiler chicks.
For this purpose, one hundred day-old chicks were divided into two groups A and
B. having fifty chicks each. Confirmed pathogenic isolate of Staphylocvccus
aureus was inoculated into the yolk of each chick of group A. while group B
acted as control group. Parameters studied included pathological examination of
yolk sac, yolk sac weight/body) weight ratio, antibody titres against Newcastle
disease virus (NDV) in serum and yolk and analysis of fractional serum proteins,
Results showed that yolk sac weight/body weight ratio was higher with marked
pathological changes (abnormal colour, consistancy and odour) were also observed
in unabsorbed yolks of the infected group. Geometric mean titres of maternal
antibodies against NDV were significantly lower in serum but higher in
unabsorbed yolks of infected group than in control one. Sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was used for serum protein
analysis and percent area covered by each protein fraction was calculated. The
percent area covered by most serum protein fractions was lower in infected
chicks than the control chicks. It was therefore concluded that yolk sac
infection (omphalitis) with Staphylocvccus aureus led to decline in the
immunity.