EFFECT OF EXPERIMENTAL YOLK SAC INFECTION WITH
ESCHERICHIA COLI ON
IMMUNE STATUS OF BROILER CHICKS
M. S. D. Shah, S. A. Khan, A. Aslam, M.
Rabbani1, K. A. Khan and M. F. Rai
Department of Pathology, 1Department of Microbiology,
University of Veterinary and Animal Sciences,
Lahore, Pakistan.
Abstract
In this study, 100 day-old broiler chicks
were divided into two groups, A and B containing 50 birds each. Experimental
infection with E. coli was given intra
yolk to group A on day-1 of experiment, while group B was kept as control.
Parameters studied were examination of yolk sac, yolk sac:body weight ratio,
antibody titer against Newcastle
disease virus (NDV) and analysis of fractional serum proteins. Results showed
that yolk sac infection with E. coli led to gross pathological changes of yolk sac (enlarged,
discoloured, changed consistency and congested blood vessels). Yolk sac:body
weight ratio was increased and geometric mean titer against NDV was decreased in
serum and yolk of treatment group. Analysis of fractional serum proteins
revealed that percentage area covered by most of serum protein fractions was
decreased in treatment group as compared to control group. It was concluded that
intra yolk infection with E. coli
resulted in pathological changes of yolk sac, increased yolk sac:body weight
ratio, decreased immunity and altered serum proteins profile of chicks.