GENETIC DIVERSITY OF HARIANA AND HISSAR CATTLE FROM
PAKISTAN
USING MICROSATELLITE ANALYSIS
M. S. REHMAN AND M. S. KHAN
Department of Animal Breeding and Genetics,
University
of
Agriculture,
Faisalabad-38040,
Pakistan
Abstract
Genetic diversity of Hariana and Hissar cattle breeds of
Pakistan
was investigated by using 30 bovine microsatellite markers proposed by the Food
and Agriculture Organization and the International Society for Animal Genetics.
The study was aimed at assessing the current genetic variability to understand
whether the two cattle breeds are genetically different.
Out of 30 loci, 27 microsatellite loci amplified well and exhibited
polymorphisms.
Polymorphic information content (PIC) value was 0.749 in Hariana and 0.719 in
Hissar. A total of 128 alleles were detected across the 27 loci with an average
of 4.59 and 4.37 alleles per locus in Hariana and Hissar breeds, respectively.
The observed number of alleles (Na) ranged from 2 (CSRM60) to 7
alleles (BM1818; ETH10) in Hissar cattle, and 3 (INRA032; MM12) to 6 (BM1818;
ETH10; INRA023; SPS115) in Hariana cattle. The effective number of alleles (Ne)
were in the range of 1.905 (HEL1) to 3.774 (TGLA126) in Hariana and 1.513
(ETH225) to 5.708 (BM1818) in Hissar cattle. The estimated mean observed (Ho)
and expected heterozygosity (He) were 0.51 and 0.67 in Hariana vs.
0.47 and 0.63 in Hissar cattle, respectively. Heterozygote deficiency analysis
revealed that both the populations exhibited significant deviations from Hardy
Weinberg (HW) equilibrium (P<0.05) at some of the loci. There was a significant
deficit of heterozygotes (FIS) in both the breeds, ranging from 25.2%
in Hariana to 25.9% in Hissar. The average FIS values for most of the
loci in both breeds were significantly different (P<0.05) from zero. These
results reveal that although Hariana and Hissar breeds shared the common
breeding tract, they are genetically different enough as separate breeds.