PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, 2009, 29(2): 72-74   next page
 

PRODUCTION OF HOMOLOGOUS LIVE ATTENUATED CELL CULTURE VACCINE FOR THE CONTROL OF PESTE DES PETITS RUMINANTS IN SMALL RUMINANTS

M. ASIM, A. RASHID, A. H. CHAUDHARY AND M. S. NOOR

Veterinary Research Institute, Ghazi Road, Lahore Cantt, Pakistan

Abstract

    Antibody response of a live-attenuated Peste des Petits Ruminants (PPR) cell culture vaccine was studied at Veterinary Research Institute, Lahore, Pakistan. For this purpose, one group of five sheep and 5 goats was each vaccinated subcutaneously with 1 ml reconstituted PPR vaccine, whereas second group of five sheep and 5 goats was inoculated with 1 ml saline solution. Blood samples were collected before and after vaccination, sera were obtained and analyzed for antibodies against PPR by competitive ELISA (cELISA). Findings suggested that antibody titres at day zero, 21 and 45 were 24.762 ± 2.69, 65.467 ± 2.29 and 83.012 ± 2.11 in sheep and 18.723 ± 2.27, 59.162 ± 1.53 and 72.176 ± 2.93 in goats. No untoward reactions were observed following vaccination. All vaccinated animals developed high titre of antibodies (PI>50).

Key words: Peste des Petits Ruminants, cell culture, vaccine, live attenuated.

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



scopus
 
DOI
 
DOAJ SEAL