Pathological Effects of Aflatoxin and Their Amelioration by Vitamin
E in White Leghorn
Layers
Wajid A Khan, M Zargham Khan*, Ahrar Khan and Iftikhar Hussain1 *Corresponding author:
mzargham2000@yahoo.com
Abstract
White Leghorn layer breeder hens, 30 weeks of age, were divided into 12 groups
(A-L). Group A was kept on basal feed and served as control, while group B was
offered feed supplemented with vitamin E (100 mg/Kg). Groups C-G were offered
feed containing 100, 500, 2,500, 5,000 and 10,000 µg/Kg aflatoxin B1 (AFB1),
respectively, whereas groups H-L were offered same dietary levels of AFB1 along
with vitamin E (100 mg/Kg). The experimental feeds were offered for three weeks
and afterward all the groups were switched over to basal feed for next two
weeks. Body weight, absolute and relative weights of liver and kidneys of AF fed
birds were significantly higher than control group. Pathological lesions in
aflatoxin (AF) fed birds included enlarged, pale and friable liver, swollen
kidneys and hemorrhages on different organs. Histopathological lesions in liver
included fatty change, congestion and hemorrhages, while in kidneys tubular
necrosis, cellular infiltration, congestion and hemorrhages were found in groups
fed AFB1 at 500 μg/Kg and higher doses. In AF fed hens, no significant
ameliorative effects of vitamin E could be observed upon AF induced decrease in
feed intake, gross pathology and histopathological alterations and organ weight
except body weights. It was concluded that the vitamin E ameliorated the AFB1
induced toxic effects in some of parameters studied.
Key words:
Aflatoxin, Aflatoxicosis, Amelioration,
Layer,
Pathological effects,
Vitamin E