The Level of Vasopressin is not solely resulted
from the Concentration of Endotoxin but Proportional to Creatinine
in Dogs with Pyometra
W. Y. Shia1, K. C. Tung1, 2,S. C. Chang1, 2,
C. H. Yang1, 2, C. H. Lee2, C. C. Chou1, and
W. M. Lee1, 2*
1Department
of Veterinary Medicine, College of Veterinary Medicine; 2
Veterinary Medicine Teaching Hospital, College of Veterinary
Medicine, National Chung Hsing University, 250-1, Kuo Kuang Rd.,
Taichung city, 402 Taiwan, R.O.C.
*Corresponding author: wmlee@dragon.nchu.edu.tw
Abstract
Pyometra is one of the most common reproductive
disorder characterized as fluid accumulation mainly pus and Gram-negative
bacteria isolated from uterus in bitches. Impaired function of vasopressin is
found in the disease. The impact of the circulating endotoxin concentration on
vasopressin involved water regulation is still unclear. To document the effect
of endotoxin on vasopressin-involved water regulation in dogs with pyometra,
blood samples were collected for examination of circulating endotoxin
concentration, osmolarity values, vasopressin concentrations, blood urea
nitrogen (BUN), and creatinine. The results indicated that the concentration
change of endotoxin was contrary to BUN and creatinine in dogs with pyometra.
The trends of concentrations change of BUN and creatinine were similar with
osmolarity and vasopressin. Pyometric dogs with high level of circulating
endotoxin had significantly lower (P<0.05) plasma osmolarity and vasopressin
concentration than those which had low levels of serum endotoxin. Plasma
osmolarity was positive correlated with both BUN and creatinine concentrations (rP
= 0.55 and 0.53, respectively; P<0.01). Likewise, the vasopressin concentration
showed good correlation with serum creatinine value (rP = 0.73; P<0.05).
Pyometric dogs with high levels of BUN (> 20 mg/dL) and creatinine (> 1.5 mg/dL) also had plasma vasopressin significantly higher
(P<0.05) than those with low levels of BUN and creatinine and control dogs. The
overall results suggested that elevated vasopressin was not mainly associated
with the circulating endotoxin concentration and some other factors may
collaborate with endotoxin causing impaired function of vasopressin.