Loop-Mediated Isothermal Amplification Assay Targeting the MOMP
Gene for Rapid Detection of Chlamydia psittaci Abortus Strain
Guo-Zhen Lin, Fu-Ying Zheng, Ji-Zhang Zhou, Guang-Hua Wang, Xiao-An
Cao, Xiao-Ei Gong and Chang-Qing Qiu*
State Key Laboratory of Veterinary Etiological Biology, Key
Laboratory of Veterinary Public Health of Ministry of Agriculture,
Lanzhou Veterinary Research Institute, Chinese Academy of
Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046,
China
Abstract
For rapid detection of the Chlamydia
psittaci (C. psittaci) abortus strain, a loop-mediated isothermal
amplification assay (LAMP) assay was developed and evaluated in this study. The
primers for the LAMP assay were designed on the basis of the main outer membrane
protein (MOMP) gene sequence of C. psittaci. Analysis showed that
the assay could detect the abortus strain of C. psittaci with adequate
specificity. The sensitivity of the test was the same as that of the
conventional nested-conventional polymerase chain reaction (PCR) and higher than
that of chick embryo isolation. Testing of 153 samples indicated that the LAMP
assay could detect the genome of the C. psittaci abortus strain
effectively in clinical samples. This assay is a useful tool for rapid diagnosis
of C. psittaci infection in sheep, swine and cattle.