Influence of different storage media, temperatures and time duration
on susceptibility of Ornithobacterium rhinotracheale
Sureerat Numee, Rüdiger Hauck, and Hafez M. Hafez*
Institute of Poultry Diseases, Faculty of Veterinary Medicine, Free
University Berlin, Königsweg 63, 14163 Berlin, Germany
*Corresponding Author: hafez@vetmed.fu-berlin.de
Abstract
Ornithobacterium rhinotracheale
(ORT) is an important respiratory pathogen of chickens and turkeys. Isolation of
the bacterium from diseased birds is necessary for serotyping, to determine the
antimicrobial susceptibility for an
effective therapy and to produce autogenous vaccines. A series of experiments
was carried out to determine optimal conditions for storage of swabs soaked in
ORT suspension. Swabs were immersed in viable ORT suspensions with different
bacterial counts and then stored under different conditions. At several time
points the viable ORT count in the swabs was determined. Dry cotton swabs as
well as three transport media, namely Amies gel medium (AG), Amies gel medium
with charcoal (AC), and Stuart gel medium (SG) were tested. ORT could be
reisolated from dry swabs stored at room temperature for up to five days and
from swabs stored in the media at room temperature for more than seven days.
Differences among the transport media were minor. The minimal number of cfu in
the ORT-suspension, in which the swabs were soaked, was 105 cfu/ml
for successful reisolation of ORT one day post immersion from swabs stored at
room temperature in AC medium, and 106 cfu/ml was successful for
reisolation from dry swabs. Higher inoculation doses and storage at 4°C
prolonged the period in which ORT could be reisolated. Storage of dry swabs at
-20°C allowed reisolation of ORT at a constant level for at least 5 d.p.i.
Inoculation of swabs with ORT and E. coli
reduced the period for which ORT could be reisolated.
Key words:
Diagnosis,
ORT,
Transport medium,
Viability