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Effects of Ochratoxin A Feeding in White Leghorn Cockerels on Hematological and Serum Biochemical parameters and its Amelioration with Silymarin and Vitamin E
M. Fakhar-ud-Din Ahmad, M. Kashif Saleemi*, M. Zargham Khan, Ahrar Khan, Faqir Muhammad1, Zahoor-ul-Hassan2, Aisha Khatoon, Sheraz Ahmed Bhatti, Rao Zahid Abbass3 and Ishtiaq Ahmed4
Department of Pathology, 1Department of Physiology & Pharmacology, University of Agriculture, Faisalabad; 2Department of Animal Sciences, KPK Agricultural University, Peshawar; 3Department of Parasitology, University of Agriculture, Faisalabad; 4Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan *Corresponding Author:


The objective of the present study was to evaluate the hematobiochemical effects tempted by ochratoxin A (OTA) in White Leghorn(WL) cockerels and to evaluate the effect of silymarin (SL), vitamin E (VE) and their combination against OTA persuaded pathological alterations in cockerels. A total of 240 day-old WL cockerels were divided into 12 groups A-L having 20 birds in each group and group A was control.  Two doses of OTA 1000 and 2000 µg/kg of feed were given to cockerels up to 7 weeks. Silymarin was administered at a dose rate of 1000 mg/kg and Vitamin E at a dose rate of 200 mg/kg alone and in combinations with two doses of OTA.  In OTA treated groups total erythrocytes counts, leukocytes count, PCV and Hb were decreased as compared to control, SL and VE groups. Albumen and serum total proteins in OTA treated groups were significantly lower as compared to control, SL and VE groups. Serum alanine transferase was significantly increased in OTA fed groups in comparison with control, SL and VE groups. Creatinine and urea were increased in OTA treated groups but were almost normal in SL and VE groups. Results showed that OTA had severe effect on liver and kidney but SL and VE treated groups had normal liver and kidneys showing its hepatoprotective effects. However, at higher dose of OTA this ameliorative effect was partially observed. These agents may be recommended as a remedy for ochratoxicosis.

Key words: Biochemistry, Cockerels, Ochratoxin A, Silymarin, Vitamin E, White Leghorn


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)