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Induction of Probiotic Strain Enterococcus faecium EF1 on the Production of Cytokines, Superoxide Anion and Prostaglandin E2 in a Macrophage Cell Line
Wei-fen Li, Qin Huang, Ya-li Li, Imran Rashid Rajput, Yi Huang§, Cai-hong Hu*
1Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal Science, Zhejiang University, Hangzhou, P.R. China, 310058*C§Also belongs to: College of Animal Science and Technology, Guangxi University, Nanning, P.R. China, 530005
*Corresponding author:


Immunological effects of probiotic strain Enterococcus faecium EF1 was evaluated through the production of cytokines, superoxide anion (O2.-) and prostaglandin E2 (PGE2) in murine macrophage cell line RAW 264.7. In addition, the responses were contrasted with a pathogenic coliform, Escherichia coli strain K88. After 12-h co-incubation of RAW 264.7 with E. faecium EF1 or E. coli K88, the culture supernatants were collected for further analysis of cytokines (TNF-α, IFN-γ, IL-1β, IL-6, IL-12, IL-8 and IL-10) and inflammatory mediators including superoxide anion (O2.-) and PGE2. The results showed that, E. faecium EF1 induced an increased (P<0.01) release of TNF-α, IFN-γ, IL-6, IL-10 and O2.- in RAW 264.7 cells. However, levels of cytokines induced by E. faecium EF1 were far lower (P<0.01) than those observed following co-culture with E. coli K88. In addition, when RAW 264.7 cells were first treated with E. faecium EF1 and then infected with E. coli K88, the production of IL-12 and O2.- in response to E. coli K88 were significantly suppressed, indicating that E. faecium EF1 may limit the ability of macrophages to induce excessive inflammation even to potent inflammatory bacteria. Our current findings concluded that, E. faecium EF1 was capable of triggering a moderate innate inflammatory response on direct contact with murine macrophage cell line RAW264.7.

Key words: Cytokine, Enterococcus faecium, Prostaglandin E2, Superoxide anion


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)