Comparative Serological and Random Amplified Polymorphic DNA Typing
for Bordetella avium
Isolates in China
Ping-Ping Yang, Rong-De Ma1, Xue Zhao and Rui-Liang Zhu*
College of Animal Science and Veterinary Medicine, Shandong
Agricultural University, Taian, Shandong 271000,
PR China;
1Linqu Animal Husbandry Bureau, Linqu, Shandong 262600,
PR China
*Corresponding author: zhurl@sdau.edu.cn
Abstract
To study the similarity among Bordetella
avium isolates in China, antigens
and diagnostic antiserum of 22 B. avium
isolates were prepared for serotyping, and a set of 20 commercially available
primers was screened out to identify suitable primers for random amplified
polymorphic DNA fingerprinting (RAPD) analysis in this study. Twenty-two
B. avium isolates were divided into
two serovars (A and B) based on their reaction in the plate-agglutination test.
Four primers R1, R2, R4 and R10 resulted in informative fingerprints and were
used to evaluate the B. avium
isolates. Based on their RAPD patterns, a dendrogram allowed the separation of
the B. avium isolates into six
genetic similarity clusters. However, no direct correlation was observed between
serotypes and RAPD typing among the isolates.