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Comparative Serological and Random Amplified Polymorphic DNA Typing for Bordetella avium Isolates in China
Ping-Ping Yang, Rong-De Ma1, Xue Zhao and Rui-Liang Zhu*
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271000, PR China; 1Linqu Animal Husbandry Bureau, Linqu, Shandong 262600, PR China
*Corresponding author:


To study the similarity among Bordetella avium isolates in China, antigens and diagnostic antiserum of 22 B. avium isolates were prepared for serotyping, and a set of 20 commercially available primers was screened out to identify suitable primers for random amplified polymorphic DNA fingerprinting (RAPD) analysis in this study. Twenty-two B. avium isolates were divided into two serovars (A and B) based on their reaction in the plate-agglutination test. Four primers R1, R2, R4 and R10 resulted in informative fingerprints and were used to evaluate the B. avium isolates. Based on their RAPD patterns, a dendrogram allowed the separation of the B. avium isolates into six genetic similarity clusters. However, no direct correlation was observed between serotypes and RAPD typing among the isolates.

Key words: Bordetella avium (B. avium), Plate-agglutination test, RAPD typing, Serotyping


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)