Single Nucleotide Polymorphism (SNP) in
PPARGC1A
Gene Associates Milk
Production Traits in
Chinese Holstein Cattle
M. A. Alim§, Yipeng Fan§, Yan Xie, Xiaoping
Wu, Dongxiao Sun*, Yi Zhang, Shengli Zhang, Yuan Zhang, Qin Zhang
and Lin Liu1
College of Animal Science and Technology, Key Laboratory of Animal
Genetics and Breeding of Ministry of Agriculture, National
Engineering Laboratory for Animal Breeding, China Agricultural
University, Beijing 100193; 1Beijing Dairy Cattle Center, Beijing
100085, China
Abstract
This study was designed to find out possible associations between
single nucleotide polymorphism (SNP)
of
PPARGC1A
gene and milk production traits. In the current study, one
SNP at g.85330C>T position was
identified in 9th intron of
PPARGC1A
gene through
pooled DNA sequencing. The identified SNP was genotyped using
MALDI-TOF MS technique in 752
individuals from the Chinese Holstein cattle breed. The frequency of C allele at
position g.85330C>T was more frequent in our population (0.69), followed by the
T allele (0.31). From the association results, significant differences were
found between
PPARGC1A
genotypes in the protein concentration and protein yield. Heterozygous cows with
CT genotype at the g.85330C>T locus showed the highest protein yield, with more
than 4.18 kg compared with homozygous cows with TT genotype; homozygous CC cows
were found at intermediate position. In case of protein percentage, homozygous
cows with TT genotype were significantly greater than heterozygous CT cows, with
a difference of about 0.012%; CC cows were found in lowest position. The allele
substitution results demonstrated that g.85330C>T-C allele decreased protein
yield (1.23kg) and protein percentage (0.016%). This result clearly indicated
that g.85330C>T-T allele increased protein percentage and protein yield.
Key words:
Chinese
Holstein,
Single nucleotide polymorphism,
PPARGC1A
gene