College of Veterinary Medicine,
Huazhong Agricultural University,
Wuhan,
China, 430070 *Corresponding author: zfxi1982@163.com
Abstract
It is believed that ascitic broilers die of right
heart failure caused by pulmonary hypertension, but the underlying mechanisms of
right heart failure are unknown. However, recent studies have shown that
reactive oxygen species have the ability to damage heart cells. This study aimed
to determine the changes of reactive oxygen species in serum and plasma, and the
effect of this variation on myocardial cells during broiler ascites. We used
hypoxia and a low-temperature method to induce broiler ascites in the
fast-growing group. For controls, we treated a slow-growing group of broilers
with 70% restricted feeding under the same circumstances as the fast-growing
group. The results showed that hypoxia is a more effective and better way to
induce broiler ascites than a low-temperature environment and high growth rate.
In addition, reactive oxygen species levels were significantly increased in the
fast-growing group compared with those in the slow-growing group. This
significant increase in reactive oxygen species resulted in myocardial cell
apoptosis in the fast-growing group. Our results suggest that cardiomyocyte
apoptosis caused by increased reactive oxygen species levels of ascitic broilers
is one of the most important reasons for causing heart failure.
Key words:
Apoptosis, Ascites,
Lipid peroxidation, Reactive oxygen species (ROS)