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Fecal Progestin Extraction and Analysis for Non-invasive Monitoring of Ovarian Cycle in Beef Cows
N. Yimer§, Y. Rosnina*, H. Wahid, M.M. Bukar, A. Malik, K.C. Yap, M. Fahmi, P. Ganesamurthi and A.A. Saharee
Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia; §Faculty of Veterinary Medicine, University of Gondar, 196 Gondar, Ethiopia


The aims of the present study were to determine presence of immunoreactive progestins in feces, correlate fecal progestins with plasma progesterone (P4) concentrations and subsequently assess the role of fecal progestins in monitoring estrous cycle in Kedah Kelantan (KK) beef cows. A total of 12 cycling cows were subjected to blood and matched fecal sampling twice a week for 9 weeks. The concentrations of plasma P4 and fecal progestins extracted using a modified technique, were determined by a P4 radioimmunoassay (RIA) kit. There was a significant positive correlation between the concentrations of fecal progestins and plasma P4 (r = 0.6, P<0.01), as tested for the whole group except one animal. High performance liquid chromatographic separation of fecal extracts and subsequent radioimmunoassay revealed presence of four immunoreactive progestins against the P4 antibody. These results imply that the non-invasive measure of fecal progestins using a DSL-3900 RIA kit can be used to monitor the ovarian activity of beef cows.

Key words: Feces, KK cows, Ovarian cycle, Progesterone, Progestin, Plasma


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)