Genesis of Hematopoietic
Tissue and Its Relation with Hemocytes of
Litopenaeus Vannamei
(Boone, 1931) (Crustacea: Decapoda)
Faiz Muhammad§,
Zhi-Feng Zhang*, Ming-Yu Shao1, Xiao-Li Shi and Muhammad
Shafi
Key Laboratory of Marine
Genetics and Breeding, Ocean University of China, Ministry of
Education, Qingdao 266003, P. R. China, §Also affiliated
with Centre of Excellence in Marine Biology, University of Karachi,
Pakistan *Corresponding author:
zzfp107@ouc.edu.cn
Abstract
The
Litopenaeus vannamei is a prime aquaculture species and has a worth
market value all over the world. In this study we investigated the genesis and
morphology of hematopoietic tissue (HPT) and types of hemocyte based on its
morphology and cytochemical feature using histological and histochemical
methods. The results revealed that HPT in
L. vannamei is not visible histologically in mysis and early two post larvae
(P1 & P2), and emerges in P3 which locates in
epigastric region. The HPT showed a continuous propagation of cells in
successive post larval stages. Four types of cells can be identified in the HPT
from adult specimen and mitotic activity is visible in the HPT. The hemocytes
have been differentiated into five types and some of these types are co-related
with the HPT cells. The cytochemical studies suggested that type I and II cells
of hemocytes are PAS positive while scanty presence of prophenoloxidase was
observed in type I cells similarly the type I cells are Sudan Black B positive
and rest of the cells showed weak activity against lipid detective stain. This
is the first effort towards genesis of HPT and its relation with circulating
hemocytes in L. vannamei.