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Antinematicidal Activity of Nicotiana tabacum L. Leaf Extracts to Control Benzimidazole-Resistant Haemonchus contortus in Sheep
Kareem Khoshnow Hamad*, Zafar Iqbal, Zia ud Din Sindhu and Ghulam Muhammad1
Department of Parasitology, 1Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan *Corresponding author:


This study was carried out to assess the efficacy of Nicotiana (N.) tabacum leaves to control benzimidazole-resistant Haemonchus (H.) contortus in sheep. Fecal egg count reduction test (FECRT) and egg hatch assay (EHA) revealed catastrophic levels of resistance in H. contortus of sheep against oxfendazole, a member of benzimidazole group of antinematicidals. Antinematicidal efficacy of N. tabacum leaves was evaluated employing EHA, adult motility test (AMT) and FECRT. There was a dose and time dependent antinematicidal activity of crude aqueous methanol extract (CAME) of N. tabacum leaves with estimated LC50 values of 0.566 and 1.91 mg ml-1 in EHA and AMT, respectively. There was, however, no significant-difference (P>0.05) in fecal egg count reduction (87.5 vs 88.6%) in sheep at low (2g kg-1 BW) and high (4g kg-1 BW) doses of CAME of N. tabacum leaves.  Administration of N. tabacum leaves at low dose (2 g kg-1 BW) did not exhibit untoward effects in animals; therefore, may be used in sheep harboring antinematicidal- resistant populations of H. contortus.

Key words: Antinematicidal resistance, Benzimidazole, Haemonchus contortus, Plant extract, Sheep, Tobacco


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)