Comparison of Streptokinase Activity from
Streptococcus mutans
using Different Substrates
Muhammad Anjum Zia*, Rana Faisal, Rao Zahid Abbas1,
Gull-e-Faran, Muhammad Kashif Saleemi2 and Junaid Ali
Khan3
Department of Chemistry and Biochemistry, University of Agriculture,
Faisalabad-38040, Pakistan; 1Department of Parasitology,
University of Agriculture, Faisalabad-38040, Pakistan; 2Department
of Pathology, University of Agriculture, Faisalabad-38040, Pakistan.
3Department of Physiology & Pharmacology,
University
of Agriculture,
Faisalabad-38040, Pakistan
Abstract
Streptokinase is a novel bacterial fibrinolytic enzyme that binds and activates
plasminogen and is produced by several species of
Streptococci.Streptococcus mutans was selected for
optimum production of streptokinase using corn steep liquor, molasses, rice
polishing and sugarcane bagass in liquid state fermentation. Substrates were
applied in different concentrations ranging from 0.1-0.8%. Maximum fibrinolytic
activity was observed by 0.3% corn steep liquor, 0.5% molasses and rice
polishing and 0.4% by sugarcane bagass. The fibrinolytic activity achieved by
fibrin clot lysis method was 5.5, 5.08, 5.16 and 4.75 units using corn steep
liquor, molasses, rice polishing and sugarcane bagass, respectively.