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Comparison of Streptokinase Activity from Streptococcus mutans using Different Substrates
 
Muhammad Anjum Zia*, Rana Faisal, Rao Zahid Abbas1, Gull-e-Faran, Muhammad Kashif Saleemi2 and Junaid Ali Khan3
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan; 1Department of Parasitology, University of Agriculture, Faisalabad-38040, Pakistan; 2Department of Pathology, University of Agriculture, Faisalabad-38040, Pakistan. 3Department of Physiology & Pharmacology, University of Agriculture, Faisalabad-38040, Pakistan

Abstract   

Streptokinase is a novel bacterial fibrinolytic enzyme that binds and activates plasminogen and is produced by several species of Streptococci. Streptococcus mutans was selected for optimum production of streptokinase using corn steep liquor, molasses, rice polishing and sugarcane bagass in liquid state fermentation. Substrates were applied in different concentrations ranging from 0.1-0.8%. Maximum fibrinolytic activity was observed by 0.3% corn steep liquor, 0.5% molasses and rice polishing and 0.4% by sugarcane bagass. The fibrinolytic activity achieved by fibrin clot lysis method was 5.5, 5.08, 5.16 and 4.75 units using corn steep liquor, molasses, rice polishing and sugarcane bagass, respectively.

Key words: Corn steep liquor, Streptococcus mutans, Streptokinase, Substrates

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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