Distribution of Various Mycotoxins in Compound
Feed, Total Mix Ration and Silage
N. Sultana, A. Rashid, I.
Tahira, H. U. Hanif1 and N. Q. Hanif
Romer Labs, Rawalpindi;
1Department of Environmental Sciences,
NationalUniversity of Science and Technology
(NUST), Islamabad, Pakistan *Corresponding Author:romerlabpk@yahoo.com
Abstract
Present study was planned to assess the spectrum
of natural occurrence of aflatoxins, zearalenone, ochratoxin A and A-B
trichothecenes in dairy feed, silage and total mixed rations.One hundred and seventy one samples were analyzed by chromatographic
technique. In cattle compound feed, there was a high incidence of aflatoxin B1
(97.31%) followed by aflatoxin B2 (50.33%), aflatoxin G1
(10.74%), aflatoxin G2 (1.47%), zearalenone (39.25%),
ochratoxin A (37.5%) and deoxynivalenol (2.96%) with average values of 29, 8,
21, 10, 862, 64 and 813 ng/g respectively. Nine samples were found tainted with
T-2 toxin (282ng/g), nivalenol (285ng/g) and fusarenon-x (1625ng/g)
respectively. However, frequency distribution showed that positive seventy-seven
(51.59%)samples found to be contaminated with aflatoxin B1 levels
higher than permissible level of European Commission (<20ng/g).For zearalenone, forty-four (32.50%) samples were tainted with levels
ranging from ≥500 to 3750ng/gi.e. higher than recommendations by European
commission (<500ng/g). In contrast to compound feed, mycotoxin analysis in
silage samples demonstrated the high prevalence of ochratoxin A (77.77 %)
followed by AFB1 (25%) with mean of 53 and 8.71ng/g respectively. A
scrutiny of mycotoxin for total mixed ration depicted that all samples were
contaminated with aflatoxin B1 and ochratoxin A with an average of 30
and 48.5ng/g respectively. As far as multi-mycotoxin co-existence is concerned,
compound feed was concurrently contaminated with two, three and four types of
mycotoxins.