Early Antibody, Cytokine and Chemokine Responses
during Echinococcus Granulosus
Infectionin Kazakh
Sheep with Cystic Echinococcosis Resistance Haplotype
Wenqiao Hui,
Muyesar Batur, Xinli Du, Shijun Ma, Junde Maand Bin
Jia*
College of Animal Science and Technology, Shihezi
University, Road Beisi, Shihezi 832003, Xinjiang, People’s Republic of China.
*Corresponding author: jiabin@shzu.edu.cn
Abstract
Our previous study showed that the haplotype of
MHC MvaIbc-SacIIab-Hin1Iab, was
associated with cystic echinococcosis (CE) resistance in Kazakh sheep. The
present study described here was designed to analyze the system immune response
in the very early stage of Echinococcus
granulosus (E. granulosus)infection in sheep with CE resistant haplotype. Fourteen healthy Kazakh
sheep were divided into two groups: seven sheep with CE resistant haplotype
referred to group A, while the others without this haplotype constituted group
B. These sheep were all experimentally infected with
E. granulosus eggs. Blood samples were
collected on day 0 (prior to infection), hours 2, 3, 4, and 9 post-infection as
well as days 1, 2, 3, and 7 post-infection, respectively. ELISA assay was used
to measure serum levels of antibodies (IgE and IgM), cytokines (Th1:TNF-α and
IFN-γ; Th2: IL-4 and IL-10) and chemokines (Th1:CXCL-9; Th2: CCL17) at different
time points of E. granulosus infection
in the two groups. Resultsshowed
that, in the early stage of E. granulosus
infection, antibodies like IgM and IgE, Th1 cytokines such as IFN-γ and
TNF-α, as well as Th1 chemokines CXCL-9 were predominant in group A, especially
for IgE and Th1 cytokines, which were significantly higher, most were at or
began from 4h post-infection, as compared with group B. Our findings revealed
that the influence of the host’s genetic background on the immunopathology of
E. granulosus infection in the early
stage could be partially mediated by Th1-type cytokines and IgE.