Distribution of Acetylcholinesterase Positive
Neurons in the Oviduct of Laying Hen
Jameel Ahmed Gandahi1,2,§,
Noor Samad Gandahi2,§, Ping Yang1, Xun
Guang Bian1, Muhammad Ghiasuddin Shah2,
Moolchand Malhi1,2,
Lin Li Zhang1,
Qian Zhang1 and Qiusheng Chen1,*
1Key
laboratory of Animal
Physiology & Biochemistry, College of Veterinary Medicine,
Nanjing Agricultural University, 210095 Nanjing-PR China; 2Faculty of Animal Husbandry and Veterinary Sciences,
Sindh Agriculture University 70060, Tandojam-Pakistan
*Corresponding author: chenqsh305@yahoo.com.cn
Abstract
The acetylcholinesterase histochemistry is used
to identify the cholinergic nerves in tissue sections. Little is known on
localization of cholinergic nerves in the oviduct of laying hens. We have used
this technique to localize and compare the acetylcholinergic neurons in
different regions through the oviduct in laying hens. The cholinergic neurons
were seen as single cells, pairs or three cells arranged together. The cytoplasm
and the processes of positive neurons showed strong reaction, with an eccentric
nucleus. Morphologically, the neurons were rounded and oval cells of unipolar,
bipolar and multipolar shapes. Similar features were seen in the whole mounts.
Varicose nerve fibers were present. Cholinergic neurons were commonly seen in
the muscularis; the fibers ran along the muscularis, occasionally showed a
bifurcation to enter the lamina propria, reaching the secondary and tertiary
mucosal folds; the fibers also targeted the blood vessels in the intermuscular
region. The regional distribution of cholinergic neurons was highest seen in the
infundibulum; medium in the magnum, isthmus and uterus (shell gland), while
vagina had significantly lower (P<0.05)number; i.e. 8.00±1.00, 5.33±0.33, 4.67±0.67; 5.67±0.33; and
3.67±0.33, respectively. The local comparison of cholinergic neurons in
muscularis and lamina propria showed significantly higher (P<0.05)number in muscularis than lamina propria of the isthmus. It was
concluded that acetylcholinesterase positive (cholinergic) nerves may have a
role in the regulation of the smooth muscle functions and blood supply in the
oviduct of chicken.