The Concentration McMaster technique is Suitable
for Quantification of Coccidia Oocysts in Bird Droppings
Jaroslav Vadlejch*1, Miloslav Petrtýl1,
Daniela Lukešová2, Zuzana Čadková1, Marie
Kudrnáčová1, Ivana Jankovská1 and Iva Langrová1
1Department
of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural
Resources, Czech University of Life Sciences Prague, Kamýcká 957,
165 21 Prague 6-Suchdol, Czech Republic; 2Department of Animal Sciences and Food Processing in
Tropics and Subtropics, Faculty of Tropical Agricultural Sciences,
Czech University of Life Sciences Prague,Kamýcká 165, 165 21 Prague 6-Suchdol, Czech Republic
Abstract
The objective of this study was to evaluate the
suitability of the Concentration McMaster technique in counting coccidia oocysts
and to compare it with two others -McMaster method modified by Wetzel and
Zajíček. A stock suspension containing
Eimeria oocysts was prepared, from which five concentrations
(20, 250, 500, 1,000 and 1,500) of oocysts were made. Each method
was evaluated after the examination of 30 samples in five concentrations. Each
sample was presented as a parasite negative fecal dropping
into whicha known numberof
oocystswereinoculated.
When results of all three methods were compared, statistically significant
differences (P<0.05) among them in each tested OPG concentration were found. The
most diverse results (P<0.01) were provided by the Wetzel and Zajíček methods.
The Wetzel method produced the least accurate results of all the evaluated
methods and in all tested oocyst concentrations. This method
was found inappropriate for counting coccidia oocysts in fecal droppings. The
Zajíček and the Roepstorff and Nansen method produced
oocyst counts similar to the true numbers. These two methods were then
evaluated using poultry fecal droppings naturally infected by
coccidial infection. Differences between these two
methods were found in the repeatability of
the obtained results rather than in the oocyst counts. Compared to the Zajíček
method, the Roepstorff and Nansen method is faster, less laborious, and produces
sensitive and reliable results with repeated measurements over
time. For all these reasons the Concentration McMaster technique
could be recommend for the detection and quantification
of coccidia oocysts in bird droppings.