Genetic Diversity in
Salmonella Isolates from Ducks and their Environments in Penang,
Malaysia using
Enterobacterial Repetitive Intergenic Consensus
Frederick Adzitey 1, Gulam Rusul
Rahmat Ali2*, Nurul Huda2 and Rosma Ahmad3
1Animal
Science Department, Faculty of Agriculture, University for
Development Studies, Box TL 1882, Tamale, Ghana; 2Food
Technology Programme, School of Industrial Technology, Universiti
Sains Malaysia, USM 11800, Penang, Malaysia; 3Bioprocess
Technology Programme,School
of Industrial Technology, Universiti Sains Malaysia, USM 11800,
Penang, Malaysia
*Corresponding author:
gulam@usm.my
Abstract
A total of 107
Salmonella isolates (37
S. Typhimurium, 26 S. Hadar, 15 S. Enteritidis, 15
S. Braenderup, and 14 S. Albany) isolated from ducks and their
environments in Penang, Malaysia were typed using
enterobacterial repetitive intergenic consensus (ERIC) to determine
their genetic diversity. Analysis of the Salmonella strains by ERIC
produced DNA fingerprints of different sizes for differentiation purposes. The
DNA fingerprints or band sizes ranged from 14-8300bp for S. Typhimurium,
146-6593bp for S. Hadar, 15-4929bp for S. Enteritidis, 14-5142bp
for S. Braenderup and 7-5712bp for S. Albany. Cluster analysis at
a coefficient of 0.85 grouped the Salmonella strains into various
clusters and singletons. S. Typhimurium were grouped into 10 clusters and
6 singletons, S. Hadar were grouped into 3 clusters and 18 singletons,
S. Enteritidis were grouped into 3 clusters and 7 singletons, S.
Braenderup were grouped into 4 clusters and 7 singletons, and S. Albany
were grouped into 3 clusters and 7 singletons with discriminatory index (D)
ranging from 0.92-0.98. ERIC proved to be a useful typing tool for determining
the genetic diversity of the duck Salmonella strains.