Microsatellite markers based genetic diversity analysis in Damani and Nachi goat breeds of Pakistan
Tanveer Hussain*1,
Masroor Ellahi Babar2,Haleema Sadia1,Misbah Shaheen1, Asif Nadeem1,
Akhtar Ali1, Abdul Wajid1 and Sajjad Ali Shah1
1Institute
of Biochemistry and Biotechnology, 2Department of Livestock
Production, University of Veterinary and Animal Sciences,
Lahore, Pakistan *Corresponding author: tanveer.hussain@uvas.edu.pk
Abstract
The genetic variation in two indigenous Pakistani
goat breeds (Damani and Nachi) was studied with 9 microsatellite markers in
order to determine the genetic diversity between them. A total number of 50 non
relative individuals of Damani (25) and Nachi (25) were sampled to explore
genetic polymorphisms and relationship between these two important goat breeds.
Result revealed considerable level of genetic diversity in both breeds and a
total number of 53 alleles were identified with mean of 3.2 in Damani and 4.6 in
Nachi. The genetic diversity in both breeds ranged from 0.51(Nachi) to 0.73 (Damani).
High level of genetic differentiation (FST= 0.20) and low level of gene flow (Nm=0.95)
found could be due to their divergent or expanded geographical locations.
Heterozygote across two populations (FIT)
was found to be 0.15. The mean Polymorphic Information Content (PIC) was 0.70
ranging from 0.54 (MAF33) to 0.83 (ILSTS011), revealed the high level of
polymorphism for studied microsatellite markers set in this study.
The measures of genetic variation revealed that there is good scope for
effective improvement, conservation and designing national breeding policies for
goat breeds in future.