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Microsatellite markers based genetic diversity analysis in Damani and Nachi goat breeds of Pakistan
 
Tanveer Hussain*1, Masroor Ellahi Babar2, Haleema Sadia1,  Misbah Shaheen1, Asif Nadeem1, Akhtar Ali1, Abdul Wajid1 and Sajjad Ali Shah1
1Institute of Biochemistry and Biotechnology, 2Department of Livestock Production, University of Veterinary and Animal Sciences, Lahore, Pakistan
*Corresponding author: tanveer.hussain@uvas.edu.pk

Abstract   

The genetic variation in two indigenous Pakistani goat breeds (Damani and Nachi) was studied with 9 microsatellite markers in order to determine the genetic diversity between them. A total number of 50 non relative individuals of Damani (25) and Nachi (25) were sampled to explore genetic polymorphisms and relationship between these two important goat breeds. Result revealed considerable level of genetic diversity in both breeds and a total number of 53 alleles were identified with mean of 3.2 in Damani and 4.6 in Nachi. The genetic diversity in both breeds ranged from 0.51(Nachi) to 0.73 (Damani). High level of genetic differentiation (FST = 0.20) and low level of gene flow (Nm=0.95) found could be due to their divergent or expanded geographical locations. Heterozygote across two populations (FIT) was found to be 0.15. The mean Polymorphic Information Content (PIC) was 0.70 ranging from 0.54 (MAF33) to 0.83 (ILSTS011), revealed the high level of polymorphism for studied microsatellite markers set in this study. The measures of genetic variation revealed that there is good scope for effective improvement, conservation and designing national breeding policies for goat breeds in future.

Key words: Genetic diversity, Goat breeds, Microsatellite markers, Pakistan

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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