Prevalence of four enteropathogens with immunochromatographic rapid test in the feces of diarrheic calves in east and southeast of Turkey
Hasan Içen1, Neval Berrin Arserim2,
Nurettin IŞIK3, Cumali Özkan4* and Abdullah
Kaya4
1Department
of Internal Diseases, 2Department of Microbiology,
Faculty of Veterinary Medicine, University of Dicle, 21180,
Diyarbakır; 3Laboratory
of Research, Diagnosis and Control of Animal Diseases, Microbiology,
21010, Diyarbakır; 4Department
of Internal Diseases, Faculty of Veterinary Medicine, University of
Yuzuncu Yil, 65080, Van, Turkey
*Corresponding author: cozkanvet@hotmail.com
Abstract
In this study, fecal specimens taken from 192
diarrheic and 14 healthy calves (2-40 days old) were examined for the presence
of bacterial and parasitic agents. Fecal samples from diarrheic calves with the
four immunochromatographic rapid tests were 92.7% positive for four
enteropathogens. The individual prevalence was 25, 21.8, 9.4 and 2.1% for
Rotavirus, Cryptosporidium
parvum, E. coli K99 and Coronavirus, respectively.
Concomitant infections caused by two agents were 15.6% for
Rotavirus+Cryptosporidium, 1.0% for Rotavirus+Coronavirus, 5.2% for
Cryptosporidium+E. coli K99, and 7.3%
for Rotavirus+E. coli K99. Besides
concomitant infections caused by three agents were 3.1% for Cryptosporidium +Rotavirus+E.coli
K99 and 1.0%, Cryptosporidium+Rotavirus+Coronavirus. In addition one calf (1.0%)
was infected by combination of four agents as Cryptosporidium, Rotavirus,
Coronavirus, and E. coli K99. The
calculated individual prevalence was 56.9% for Rotavirus, 47.8% for
C. parvum, 26.0% for
E. coli K99 and 5.2% for Coronavirus. However, 88 samples were positive
in smear detection for Cryptosporidium while 92 were positive in rapid test.
As a result of this study it can be concluded that
multiple etiologies of diarrhea can be seen and this can help in the development
of a specific treatment and
preventative measures for practitioners in east and southeast of Turkey.