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Molecular detection of Clostridium perfringens type D alpha and epsilon toxin genes from various tissues in lambs
 
Azam Ali Nasir1,3, Muhammad Younus2,*, Muti-ur-Rehman3, Muhammad Latif3, Asif Rashid1, Rashid Ahmad1 and Muhammad Abbas1
1Veterinary Research Institute, Zarrar Shaheed Road, Lahore Cantt-54810; 2Department of Pathobiology, College of Veterinary and Animal Sciences, Jhang (sub-campus of UVAS, Lahore); 3Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan
*Corresponding author: younusrana@hotmail.com

Abstract   

Different tissues including duodenal scrapings, liver, lungs and kidneys of each infected (n=6) and control (n=4) lambs were subjected to PCR for the identification of alpha and epsilon toxin genes of Clostridium perfringens type D. Alpha and epsilon toxin genes were amplified at annealing temperature 52.2 and 50.2oC with amplicon size about 247bp and 665bp, respectively. A high percentage of alpha toxin genes of C. perfringens type D were amplified from duodenal scrapings followed by liver, kidneys lungs in cultured broth while a low percentage was observed when PCR was attempted directly on these tissues without prior culturing in media. It was concluded that distribution of alpha and epsilon toxin genes varies in different organs being the highest in duodenal tissue and PCR is suitable assay for the typing of C. perfringens toxin genes.

Key words: Alpha, Clostridium perfringens type D, Epsilon toxin genes, Lamb, PCR

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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