Co-infection of avian leukosis virus and Salmonella pullorum with the preliminary eradication in breeders of Chinese local “Shouguang” chickens
Jian Qiang Huang, Jing Kai
Xin, Cui Mao, Feng Zhong and Jia Qian Chai*
Key Laboratory of Animal
Biotechnology and Disease Control and Prevention of Shandong
Province, College of Veterinary Medicine,
Shandong
Agricultural University, Taian 271018, People’s Republic of China
*Corresponding author: jqchai@sdau.edu.cn
Abstract
The study was designed to
investigate the infection status and finish the preliminary eradication of avian
leukosis virus (ALV) and salmonella pullorum (SP) in breeders of Chinese local “ShouGuang”
chickens. ALV antigen and antibody was tested via ELISA, and SP antibody was
detected by SPAT (serum plate agglutination test). The pathology and etiology
research was also researched. The ALV-P27 antigen, ALV-A/B and SP antibody
positive chickens were eliminated in turn, and then the negative were retained
as the breeder flocks. The results showed that the positive rate of antigen to
ALV-P27, antibody to ALV-A/B, ALV-J and SP was 57.8, 6.7, 0 and 17.8% in this
breeder farm, respectively. The co-infection of ALV and SP was confirmed and the
positive rate of both SP and ALV-P27 or ALV-A/B was 10 and 1%, respectively.
There were obvious tumor nodules and lymphoid tumor cells in the comb, liver and
spleen of the co-infected chickens. The degenerative and atrophic ovarian
follicles, inflammatory cell infiltration in muscle biopsies were also found.
The elimination rate of ALV-p27, ALV-A/B and SP positive chickens was 55.4, 13
and 6.1%, respectively. The final amount of the breeder conservation was 309
chickens. In conclusion, the co-infection of ALV-B and SP was found and more
emphasis should be given on its prevention; the preliminary eradication of “ShouGuang”
breeder chickens was finished.