Estrus Synchronization and Conception Rates Using Locally Prepared Methylacetoxy-progesterone Sponges in Cyclic and Acyclic Nili-Ravi Buffaloes (Bubalus bubalis)
R Kausar*, SA Khanum, M Hussain, T
Hussain, N Ahmad1, L Ahmad2 and NA Qureshi2
Animal Sciences Division, Nuclear Institute for
Agriculture and Biology (NIAB), Faisalabad; 1Department
of Theriogenology, University of Agriculture, Faisalabad; 2Department
of Wildlife and Fisheries, Government College University,
Faisalabad, Pakistan. *Corresponding author: rehana_niab@yahoo.com
Abstract
A study was conducted to evaluate the efficacy of
locally prepared intra-vaginal methylacetoxyprogesterone (MAP) sponges for
estrus synchronization and conception rate in cyclic and acyclic Nili-Ravi
buffaloes. In the first experiment, 20 buffaloes were divided into treated and
control groups (n: 10 each) and again sub-grouped into cyclic (n: 4) and acyclic
(n: 6) lots in each group, depending on their ovarian status. MAP sponges were
placed in the vagina of buffaloes for 16 days. After sponge removal, buffaloes
were monitored for estrus. Serum progesterone concentrations were determined in
synchronized animals for one estrous cycle using radioimmunoassay. Overall,
synchronization rate in MAP-treated groups was significantly higher (P<0.05) as
compared to controls (60 vs. 0%). However, it differed non-significantly between
cyclic and acyclic buffaloes of treated groups (75 vs. 50%). At estrus, mean
serum progesterone in synchronized animals was at basal level (0.26±0.08 and
0.32±0.27 ngml-1 in cyclic and acyclic treated groups, respectively).
It attained peak levels of 3.00±1.80 to 3.45±0.44 ngml-1 on d-14,
then dropped on d-18 and declined to basal values at the next estrus. In the
second experiment, the effect of a MAP-based treatment was compared with an
11-days apart double injection prostaglandin (PGF2α) treatment for
synchronization of estrus and conception rates. The estrus synchronization and
conception rates obtained by PGF2α protocol were non-significantly
higher than those of MAP-based protocol (90 vs. 70% and 44.44 vs. 42.86%). The
synchronization and conception rates of cyclic buffaloes (80 & 50%,
respectively) receiving MAP-based regimen were also non-significantly higher
from acyclic ones (50 & 33%, respectively) receiving the same protocol. In
conclusion, the treatment with locally prepared MAP sponges proved effective in
synchronizing estrus and comparable with prostaglandin regimen in terms of
conception rates in Nili-Ravi buffaloes.