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Molecular Characterization of Staphylococcus aureus Isolated from Meat and Their Antibiotic Resistance Profiles
Ziad W Jaradat1, Yaser H. Tarazi§2 and Qotaibah O. Ababneh3
1Department of Biotechnology and Genetic Engineering, Faculty of Science and Arts, 2Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P. O. Box 3030 Irbid, 22110, Jordan;  3Department of Biophysics and Biochemistry, Texas A&M University, College Station, TX, USA *Corresponding author:


The aims of this study were to characterize S. aureus isolates from different meat sources in Jordan and study their genetic relationship using PCR-RFLP in addition to their antibiotic resistance profiles. Thirty S. aureus isolates were identified and confirmed by PCR techniques. The isolates from goat and camel meats were sensitive to the majority of the tested antibiotics. Plasmid profiling revealed that 26 isolates contained at least one plasmid with no correlation between the number of plasmids and the resistance profiles. PCR-RFLP of the coagulase gene (coa) classified the isolates to several clusters upon digestion with Alu I or Cfo I restriction enzymes. This study concluded that the 30 S. aureus isolates were genetically diverse and comprised heterogeneous population with 7 genotypes at both 33.1 and 51.2 similarity levels.

Key words: Antibiotics, Coagulase gene, Meat, RFLP, S. aureus


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)