Department of
Radiology, Seoul National University College of Medicine, Seoul
110-744; 1College
of Veterinary Medicine, Konkuk University, Seoul 143-751; 2Veterinary
Clinical Science, College of Veterinary Medicine, Chonbuk National
University, Jeonju 561-756, Republic of Korea *Corresponding author:kclee@jbnu.ac.kr
Abstract
To compare characteristics of computed
tomographic images of hepatic tumors induced by intra-mesenteric venous and
intraparenchymal injection of VX2 carcinoma in rabbits. Thirty two New Zealand
White rabbit were divided into two groups; group I for metastatic tumor model
(n=8) by injection of 0.1 ml tumor homogenate into mesenteric vein, and group II
for solitary tumor model (n=24) by direct intraparenchymal injection of 1 mm
tumor cubes. Dual-phase computed tomography (CT) was performed on day 10, 17,
and 24 post-tumor implantation. On day of each CT follow-up, 2 or 3 rabbits in
both groups were sacrificed for histopathologic examinations which were
correlated with CT findings. Tumor creation rates were 100% in Group I, and 87.5% in Group II. In
group I, arterial phase showed multiple tiny lesions on day 10, and hypodense
lesions with peripheral rim enhancement and central hyperdensity (target
appearance) on day 17 and 24 post-implantation. Target appearance of group I was
more distinctive on portal phase, and was correlated with the viable tumor
tissue core surrounded by cystic spaces in histopathologic findings. In group
II, arterial phase revealed ill-defined hypodense lesions with peripheral rim
enhancement during entire follow-up periods, and rim enhancement was disappeared
on portal phase. Viable tumor cells in group II were mainly found in periphery
of tumor with apparent central necrosis, and these observations were well
correlated with CT findings. This study showed specific characteristics
corresponding to each implantation method, and will provide beneficial
information for experimental studies using hepatic VX2 carcinoma.