Liu Xiao1,2, Wang Shu-Ying1,*,
Huang Li-Bo1, Hou Yan-Meng1 and Shi Yun-Zhi1
1College
of Animal Science and Veterinary Medicine, ShanDong Agricultural
University, Tai’an 271000, PR China; 2Shenzhen Bay
Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518000, PR
China *Corresponding author: sywang@sdau.edu.cn
Abstract
ImmunohistochemicalStrept Avidin Biotin-Peroxidase Complex (SABC)
three-step method was used to investigate the distribution patterns and
developmental changes of GnRH and GnRHR immunopositive (GnRH-ip and GnRHR-ip)
cells in the pituitary of Ji Ning Gray goats during 0-180 days of age. The
results showed that GnRH-ip and GnRHR-ip cells were detected only in the pars
distalis of adenohypophysis. There were no positive cells in the pars intermedia
and neurohypophysis. GnRH-ip and GnRHR-ip cells were not observed in the
anterior pituitary at birth day and 30 days of age. At 60 days, a number of
GnRH-ip and GnRHR-ip cells were found in the anterior pituitary. GnRH-ip cells
were pale brown which scattered or clustered around the sinusoid capillary;
GnRHR-ip cells were brown and the cytomembrane was darker. The size and
percentage of GnRH-ip and GnRHR-ip cells increased with the age growth. The
numbers of GnRH-ip and GnRHR-ip cells after sexual maturity were significantly
bigger than that before sexual maturity. The results above suggested that GnRH
and GnRHR in the pituitary of Ji Ning Gray goats play a pivotal role in the
sexual development and sexual maturity.
Key words: GnRH, GnRHR, Immunohistochemistry, Ji Ning Gray
goat, Pituitary