1College of Veterinary
Medicine, Kyungpook National University; 2Korea Institute
of Radiological and Medical Sciences; 3Chonnam National
University, College of Veterinary Medicine; 4Chungnam
National University, Gongneung-Dong, Nowon-ku, Seoul, Republic of
Korea *Corresponding author:thkim56@knu.ac.kr
Abstract
The purpose of this paper is to establish the
cytogenetic analyses of human peripheral blood samples caused by simulation of
partial-body exposures. Either accidental or occupational partial-body exposure
to ionizing radiation poses significant health hazards that are indicated by
induction of chromosome aberrations (CA). The percentages of mixtures of blood
samples irradiated in vitro with 2 Gy of gamma rays were 10, 25, 50, 75
and 100.0%. Lymphocytes were cultured for 48 hr, harvested with standard
procedures and then first-division metaphase cells were analyzed. It showed that
the frequencies of unstable CA depend on the proportion of the irradiated blood.
All frequencies of the observed CA was lower than that of predicted or
calculated from 100% exposed blood, except in one case, indicating a phenomenon
of “dilution” of the un-irradiated into irradiated lymphocytes that may take
place a bystander effects. Our data showed that the quantification of CA in
human peripheral blood lymphocytes may be an important tool of dose assessment
for partial-body exposure to ionizing radiation.