College of Animal Science, Henan Institute of
Science and Technology, Xinxiang, Henan Province, P.R. China,
453003.
*Corresponding author: wangzl_2008@yahoo.com.cn
Abstract
The objective of this study was to investigate the
effects of cadmium on DNA damage of lung cells in immature animals. Seventy-two
immature mice were randomly divided into twelve cadmium-ingested groups including low dose (1/100 LD50, 1.87
mg/kg BW), middle dose (1/50 LD50, 3.74 mg/kg BW), high dose (1/25 LD50,
7.48 mg/kg BW) and control group, and exposed to cadmium chloride for 10, 20 and
30 days, respectively. Mice were sacrificed after cadmium exposure for different
time, and
lung cells were collected to investigate DNA damage by comet assay. The results
showed that comet tailing ratio, tail length, comet length, tail moment, Olive tail moment and damaged grade of lung cells from immature mice
increased along with increasing of cadmium exposure dose and time. In low dose
group treated for 30 days, there was significance (P<0.05) in comet length or high significance (P<0.01) in other
parameters compared with control group or low dose group treated for 10 days.
When mice were exposed to cadmium at high dose for 30 days, DNA of lung cells
was damaged most seriously. Our results indicate that cadmium can induce DNA
damage of lung cells from immature mice in dose- dependent and time-dependent
manners, and DNA will be damaged when immature mice exposed to cadmium for long
time even at low dose. Meanwhile, comet assay can be considered as a powerful
and sensitive biomarker assay in risk assessment of immature animals exposed to
cadmium.