Hemato-Biochemical Effects of Colistin Administered Intramuscularly in
Growing Broiler Birds
Muhammad Kashif
Saleemi*, Muhammad Fayyaz Ul Hussan, Muhammad Zargham Khan, Ahrar
Khan, Rao Zahid Abbas1 and Arfan Ahmad2
Department of Pathology; 1Department
of Parasitology, University of Agriculture, Faisalabad 38040,
Pakistan; 2Diagnostic Laboratory, University of
Veterinary and Animal Sciences, Lahore Pakistan *Corresponding author: drkashif313@yahoo.com
Abstract
The current study was designed to probe
pathological effects of colistin in growing broilers. One hundred twenty day-old
broiler chicks were divided into six equal groups A-F. These birds were injected
colistin sulphate, 0, 5, 10, 20, 40 and 80 mg/kg BW respectively through
intramuscular route. The different serum-biochemical parameters including total
protein, albumen, globulin, ALT, AST, LDH, GGT, creatinine and BUN were
determined at day 21 and 42 of experiment. The hematological parameters
including RBC, WBC, PCV and Hb were determined twice. The serum total protein
values were significantly higher in group E as compared with control group. At
day 42 groups, C-E had significantly lower albumen values as compared to group
A. The similar pattern was observed in globulin values. The creatinine & urea
was also found significantly higher in group D and E at day 21 of experiment,
while at day 42 all the groups having significantly higher urea level in
comparison with group A indicating nephrotoxic effect of colistin. The serum
enzymes including ALT and AST were significantly higher in group D & E as
compared with group A, which is an indication of liver damage. The LDH and GGT
values showed significantly increasing trend in experimental groups compared
with control. The erythrocytes and leucocytes count were significantly higher in
control and low dose groups as compared with higher colistin dose groups. It is
concluded that lavish parenteral administration of colistin resulted in
increased levels of hepato-renal serum indicators and lower values of
hematological parameters.